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(Seminar)Maximum entropy and population heterogeneity in continuous cell cultures
2019-07-31     Text Size:  A

CAS Key Laboratory of Theoretical Physics

Institute of Theoretical Physics

Chinese Academy of Sciences

Seminar

Title

题目

Maximum entropy and population heterogeneity in continuous cell cultures 

Speaker

报告人

Prof. Robert Mulet

Affiliation

所在单位

University of Havana, Cuba

Date

日期

2019年7月31日,11:00-12:00

Venue

地点

ITP South Building 6420

Abstract

摘要

Continuous cultures of mammalian cells are complex systems displaying hallmark phenomena of nonlinear dynamics, such as multi-stability, hysteresis, as well as sharp transitions between different metabolic states. In this context mathematical models may suggest control strategies to steer the system towards desired states. Although even clonal populations are known to exhibit cell-to-cell variability, most of the currently studied models assume that the population is homogeneous. To overcome this limitation, we use the maximum entropy principle to model the phenotypic distribution of cells in a chemostat as a function of the dilution rate. We consider the coupling between cell metabolism and extracellular variables describing the state of the bioreactor and take into account the impact of toxic byproduct accumulation on cell viability. We present a formal solution for the stationary state of the chemostat and show how to apply it in two examples. First, a simplified model of cell metabolism where the exact solution is tractable, and then a genome-scale metabolic network of the Chinese hamster ovary (CHO) cell line. Along the way we discuss several consequences of heterogeneity, such as: qualitative changes in the dynamical landscape of the system, increasing concentrations of byproducts that vanish in the homogeneous case, and larger population sizes.

Contact Person

所内联系人

周海军
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