
(Seminar)Maximum entropy and population heterogeneity in continuous cell cultures 
20190731
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CAS Key Laboratory of Theoretical Physics

Institute of Theoretical Physics

Chinese Academy of Sciences

Seminar

Title
题目

Maximum entropy and population heterogeneity in continuous cell cultures 

Speaker
报告人


Affiliation
所在单位

University of Havana, Cuba 
Date
日期

2019年7月31日，11:0012:00 
Venue
地点

ITP South Building 6420

Abstract
摘要

Continuous cultures of mammalian cells are complex systems displaying hallmark phenomena of nonlinear dynamics, such as multistability, hysteresis, as well as sharp transitions between different metabolic states. In this context mathematical models may suggest control strategies to steer the system towards desired states. Although even clonal populations are known to exhibit celltocell variability, most of the currently studied models assume that the population is homogeneous. To overcome this limitation, we use the maximum entropy principle to model the phenotypic distribution of cells in a chemostat as a function of the dilution rate. We consider the coupling between cell metabolism and extracellular variables describing the state of the bioreactor and take into account the impact of toxic byproduct accumulation on cell viability. We present a formal solution for the stationary state of the chemostat and show how to apply it in two examples. First, a simplified model of cell metabolism where the exact solution is tractable, and then a genomescale metabolic network of the Chinese hamster ovary (CHO) cell line. Along the way we discuss several consequences of heterogeneity, such as: qualitative changes in the dynamical landscape of the system, increasing concentrations of byproducts that vanish in the homogeneous case, and larger population sizes.

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