|
RNA-sequencing to map transcription starts and RNA processing in bacteria |
2015-12-07
Text Size:
A A A |
Institute of Theoretical Physics | Chinese Academy of Sciences | State Key Laboratory of Theoretical Physics | Seminar | | Title 题目 | RNA-sequencing to map transcription starts and RNA processing in bacteria | Speaker 报告人 | Professor Erik Aurell | Affiliation 所在单位 | Royal Institute of Technology (KTH), Stockholm, Sweden | Date 日期 | 15:30, Dec 07 (Monday), 2015 | Venue 地点 | ITP New Building 6420, 理论物理研究所新楼四层多媒体教室 | Abstract 摘要 | I will describe a method to separately investigate primary from processed RNA in bacteria by differentially ligating short sequences, or "tags", before RNA-sequencing. The method has made possible the mapping of many transcription start sites in the human pathogen Enterococcus faecalis and the discovery of complex transcriptional regulation and some forms of post-transcriptional modifications. The method is also useful as a reliable means to discover antisense transcript which may otherwise be confounded by artifacts of RNA-sequencing. Optimal tag design led us to the problem of finding minimal absent words (MAW) in genome sequences. A probabilistic model of reading words from a random text explains the distrubution of short MAWs while the distribution of long MAWs reflects biological differences from viruses and bacteria to complex multi-cellular organisms. | Contact person 所内联系人 | Hai-Jun Zhou(周海军) |
|
|
|
|